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GrainGenes Reference Report: PRT-13-155

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Reference	PRT-13-155
Title	A reporter gene under the control of tms or aux promoters is differently expressed in tobacco and barley protoplasts
Journal	Plant Cell Reports
Year	1994
Volume	13
Pages	155-158
Author	Gaudin V Lutticke S Jouanin L
Abstract	Agrobacterium tumefaciens and some Agrobacterium rhizogenes strains possess auxin biosynthesis genes (tms and aux genes respectively), responsible for a de novo auxin biosynthetic pathway in transformed plant cells A comparison is presented of the potential expression of these genes in a monocotyledonous (barley) and a dicotyledonous plant (tobacco) The promoters of the genes were translationally fused to the beta-glucuronidase reporter gene and analysed in transient expression experiments The tms and aux fusions were highly expressed in tobacco, but not in barley. However, the aux enhancer active in tobacco, conferred low beta-glucuronidase expression in barley when fused to a truncated cauliflower mosaic virus 35S promoter. The results are discussed in relation to the differential responses to Agrobacterium infection in monocots and dicots.
Keyword	agrobacterium-rhizogenes [ Show all 18 ]

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