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GrainGenes Reference Report: PRT-14-418

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Reference	PRT-14-418
Title	Identification of a wheat (Triticum aestivum) cell line lacking a specific divalent cation requirement
Journal	Plant Cell Reports
Year	1995
Volume	14
Pages	418-422
Author	Lazar M Simmons J
Abstract	A wheat (Triticum aestivum L. cell line, derived from anther culture of an F1 hybrid, has exogenous Ca2+, to that of calcium-dependent cells grown on complete medium The calcium-independent cell line has been grown in the absence of Ca2+ for more than 1.5 years The cell line grew at a rate similar to that on complete medium for up to 12 weeks, if supplied with any one of the divalent cations, Ca2+, Mg2+, Mn2, Zn2+, Cu2+ or Co2+, but declined and appeared necrotic when all 6 of these were removed from the medium The calcium-independence trait, while identified in tissue culture, was also observed in germinated immature embryos of the same hybrid and one of its parental inbred lines
Keyword	calcium [ Show all 10 ]

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