GrainGenes Reference Report: PRT-19-1000
Reference
PRT-19-1000
Title
Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants
Journal
Plant Cell Reports
Year
2000
Volume
19
Pages
1000-1005
Author
Nobre J Davey MR Lazzeri PA Cannell ME
Abstract
Summary: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker uidA gene, and pCaIneo, which contains the selectable marker neomycin phosphotransferase gene. Protoplast-derived calluses were selected on medium containing the antibiotic G418 (25 and 15 mg.l-1) and macroscopic antibiotic resistant colonies were recovered. Fertile plants were regenerated from a callus line and molecular analysis confirmed transgene integration
Keyword
barley
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GrainGenes Reference Report: PRT-19-1000
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||