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GrainGenes Reference Report: PYC-46-23

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Reference	PYC-46-23
Title	Varietal differences and chromosome locations of multiple isoforms of starch branching enzyme in wheat endosperm.
Journal	Phytochemistry
Year	1997
Volume	46
Pages	23-26
Author	Nagamine T Yoshida H Komae K
Abstract	Starch branching enzyme isolated from wheat endosperm was fractionated into two distinct isoforms, BE I and BE II, using anion-exchange column chromatography on Q-Sepharose Fast Flow. Both BE I and BE II were separated precisely into several isoforms using native polyacrylamide electrophoresis coupled with enzyme activity staining, and varietal differences in the major BE I isoforms expressed in the endosperm 21 days after flowering were observed. Chromosome locations of genes controlling the expression of the three BE I isoforms of 'Chinese Spring' were determined using aneuploid analysis, which indicated that two isoforms, BE I-1 and BE I-2, are controlled by 1DL and the other isoform, BE I-4, is 7BL.
External Databases	https://doi.org/10.1016/S0031-9422(96)00823-0
Gene	SbeI1 (Triticum) SbeI2 (Triticum)
Gene Set	SbeI SbeII

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