GrainGenes Reference Report: RFM-26-37
Reference
RFM-26-37
Title
Differentiation of isolates of Fusarium graminearum by pathogenicity and PCR
Journal
Revista Fitotecnia Mexicana
Year
2003
Volume
26
Pages
37-42
Author
Leyva Mir SG
[ Show all 6 ]
Abstract
To differentiate wheat (Triticum aestivum) strains based on their resistance to the fusarium head scab pathogen (Fusarium graminearum (Gibberella zeae)), we evaluated 10 strains of F. graminearum collected in the States of Mexico, Michoacan, Jalisco, Tlaxcala and Puebla, from spikes of 6 different genotypes, i.e. Buc/Alucan, Chil/Chum 18 and Milan (tolerant) and Galvez, Pavon and Zacatecas (susceptible). Spike inoculation was carried out with single conidia isolates under greenhouse conditions. There were significant differences among isolates based on spike pathogenicity. The isolate from Juchitepec, Mexico was the most pathogenic, and the one from Tepatitlan, Jalisco was the least pathogenic. Genotypes Buc/Alucan, Chil/Chum 18 and Milan did not behave as resistant. The DNA analyses among isolates were made by the RAPD-PCR technique based on a 0.82 genetic similar coefficient. Five different groups were detected among isolates, which were not related to their pathogenicity in field conditions
Keyword
conidia
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GrainGenes Reference Report: RFM-26-37
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||