GrainGenes Reference Report: SCJ-17-273
Reference
SCJ-17-273
Title
Genetic diversity of Secale L. based on ISSR markers
Journal
Southwest China Journal of Agricultural Sciences
Year
2004
Volume
17
Pages
273-277
Author
Shang H Zheng Y Wei Y Wu W Yan Z
Abstract
The genetic diversity of 16 Secale spp. accessions, representing 4 species (S. strictum, S. sylvestre, S. vavilovii and S. cereale) and 8 subspecies (S. strictum subsp. anatolicum, S. strictum subsp. kuprijanovii, S. cereale subsp. tetraploidum, S. cereale subsp. ancestrale, S. cereale subsp. dighoricum, S. cereale subsp. segetale, S. strictum subsp. strictum and S. cereale subsp. cereale) was evaluated using intersimple sequence repeat (ISSR) markers. Thirty-five ISSR primers were used for the PCR amplification of genomic DNA, and 7 primers (20%) could produce clear and polymorphic bands. A total of 269 bands were amplified from 7 primers, of which 229 bands (approx equal to 85.1%) were polymorphic. Seventeen to 70 polymorphic bands could be amplified from each primer, with an average of 32.7 bands. The mean genetic similarity value based on ISSR markers among the 16 accessions was 0.732, ranging from 0.478 to 0.935. Cluster analysis showed that all materials could be classified into 5 subgroups, and S. sylvestre was clearly distinct from the other species. These results suggested that ISSR markers could be used as an effective molecular technique for the diversity and systematics study of Secale spp
Keyword
cluster analysis
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: SCJ-17-273
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||