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GrainGenes Reference Report: TAG-76-728

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Reference	TAG-76-728
Title	Genome specific highly repeated sequences of Hordeum-vulgare cloning sequence and squash dot test
Journal	Theoretical and Applied Genetics
Year	1988
Volume	76
Pages	728-732
Author	Junghans H Metzlaff M
Type	Article
Language	English
Abstract	Highly repeated sequences of nuclear DNA from barley Hordeum vulgare (L.) variety 'Erfa' were cloned. Several clones containing barley specific repeated DNA were analysed by sequence analysis and Southern blot hybridization. The investigated repeats differ from each other in their length, sequence and redundancy. Their length ranges from 36 bp to about 180 bp. The repeats are AT-rich and differ widely in their redundancy within the barley genome. Southern analysis showed that the repeats belong to different repetition complexes. The possibility for utilizing these clones as probes for simple and fast genome analysis is demonstrated in squash dot experiments.
Probe	MHV11 MHV16 MHV47 MHV56
Keyword	DNA sequence Genome characterization Molecular sequence data

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