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GrainGenes Reference Report: TAG-87-821

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Reference	TAG-87-821
Title	Molecular analysis of plants regenerated from embryogenic cultures of wheat (Triticum aestivum L.)
Journal	Theoretical and Applied Genetics
Year	1994
Volume	87
Pages	821-828
Author	Chowdhury M Vasil I
Abstract	Total DNAs of plants regenerated from immature embryo-derived 2-month-old embryogenic calli of wheat (cultivars Florida 302, Chris, Pavon, RH770019) were probed with six maize mitochondrial genes atpA, atp6, apt9, coxI, coxII, rrn18-rrn5), three hypervariable wheat mitochondrial clones K', K3, X2), five random pearl millet mitochondrial clones (4A9,4D1, 4D12,4E1, 4E11) and the often-used wheat Nor locus probe (PTA71), in order to assess the molecular changes induced in vitro In addition, protoplast-derived plants, and 24-month-old embryogenic and non-embryogenic calli and cell suspension cultures of Florida 302 were also analyzed. No variation was revealed by the wheat or millet mitochondrial clones. Qualitative variation was detected in the nonembryogenic suspension culture by three maize mitochondrial genes (coxI, rrn]8-rrn5, atp6). A callus-specific 3.8-kb HindIII fragment was detected in all four cultivars after hybridization with the coxI gene. The organization of the Nor locus of the plants regenerated from Florida 302 and Chris was stable when compared to their respective control plants and calli. The Nor locus in regenerants of Pavon and RH, on the other hand, was found to be variable. However, Nor locus variability was not observed in 14 individual seed-derived control plants from either Pavon or RH sources. In Pavon, a 3.6-kb TaqI or a 5.6-kb BamHI + EcoRI fragment was lost after regeneration. In one of the RH regenerants, which lost a fragment, an additional fragment was observed.
Keyword	embryo-culture [ Show all 10 ]

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