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GrainGenes Reference Report: TAG-91-1048

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Reference	TAG-91-1048
Title	Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	1048-1053
Author	Iqbal M Rayburn A
Abstract	The introgression of rye DNA into the wheat genome was studied using random decamer and specific primers with the polymerase chain reaction (PCR) DNA from paired near-isolines in Chisholm and Arkan backgrounds differing with respect to the presence of a 1 RS.1 BL translocation was amplified with 120 arbitrary sequence primers Two of the primers (OPR 19 and OPJ07) amplified rye-specific DNA fragments The OPR19 primer amplified a 1.35-kb fragment that appeared to be specific to the 1 RS.1 BL translocation, based on its presence only in lines carrying the 1 RS.1 BL translocation. A fragment of the same size was also amplified in 1 RS.1 AL translocation lines. This 1 RS.1 BL marker locus was designated Ximc 1. The other primer, OPJ07, amplified a 1.2-kb DNA sequence, that was designated Ximc 2, specific to the wheat-rye translocation in various wheat backgrounds. The sequences of the two marker loci were found to be different from each other. The Ximc 1 locus was a low-copy sequence which was also present in Balboa rye genomic DNA. Through the use of specific primers, the presence of the rye-specific marker was confirmed in hexaploid as well as in tetraploid wheat backgrounds. The use of RAPDs for the study of smaller alien introgressions into wheat is discussed.
Keyword	chromosome [ Show all 14 ]

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