GrainGenes Reference Report: TAG-98-1
Reference
TAG-98-1
Title
Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes.
Journal
Theoretical and Applied Genetics
Year
1999
Volume
98
Pages
1-9
Author
Taketa S Harrison GE Heslop-Harrison JS
Abstract
The physical locations of the 5S and 18S-25S rDNA sequences were examined in nine wild Hordeum species and cytotypes by double-target in situ hybridization using digoxigenin-labelled 5S rDNA and biotin-labelled 18S-25S rDNA as probes. H. vulgare ssp. spontaneum (2n = 2x = 14; I-genome) had a similar composition of 5S and 18S-25S rDNA to cultivated barley (H. vulgare ssp. vulgare, I-genome), with two major 18S-25S rDNA sites and minor sites on four of the other five chromosomes; three chromosomes had 5S rDNA sites. The closely related H. bulbosuin (2x; also I-genome) showed only one pair of 5S rDNA sites and one pair of 18S-25S rDNA sites on different chromosomes. Four wild diploid species, H. marinum (X-genome), H. glaucum and H. murinum (Y-genomes) and H. chilense (H-genome), differed in the number (2-3 pairs), location, and relative order of 5S and the one or two major 18S-25S rDNA sites, but no minor 1S-25S rDNA sites were observed. H. murinum 4 had three chromosome pairs carrying 5S rDNA, while the diploid had only a single pair. Two other tetraploid species, H. brachyantherum 4x and H. brevisubulatum 4x (both considered to have H-type genomes), had minor 18S-25S rDNA sites, as well as the major sites. Unusual double 5S rDNA sites -- two sites on one chromosome arm separated by a short distance -- were found in the American H-genome species. H. clilense and H. brachyantherum 4x. The results indicate that the species H. brachyantherum 4x and H. brevisubulatum 4x have a complex evolutionary history, probably involving the multiplication of minor rDNA sites (as in H. vulgare sensu lato), or the incorporation of both I and H types of genome. The rDNA markers are useful for an investigation of chromosome evolution and phylogeny.
External Databases
Agricola: 1999-07860 http://link.springer.de/link/service/journals/00122/bibs/9098001/90980001.htm
Keyword
in situ hybridization karyotype evolubion rdna ribosomal dna wild relatives
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-98-1
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||