GrainGenes Reference Report: TAG-100-569
Reference
TAG-100-569
Title
Intraspecific genetic variability analysis of Neovossia indica causing Karnal bunt of wheat using repetitive elements
Journal
Theoretical and Applied Genetics
Year
2000
Volume
100
Pages
569-575
Author
Datta R
[ Show all 6 ]
Abstract
Summary: Neovossia indica (Tilletia indica), causing Karnal bunt of wheat, affects major wheat growing regions all over the world. Karnal bunt ranks as one of the major diseases of wheat causing quality losses and monetary losses due to international quarantine regulations. The present work is the first report of a genetic diversity analysis of Indian isolates of N. indica. A library of N. indica isolate Ni7 was constructed in a lambda ZAPII system, and three repetitive elements were identified for molecular analysis. These repetitive elements generated complex hybridization profiles producing fingerprint patterns of all seven isolates. Copy-number estimation of these three elements, pNiR9, pNiR12 and pNiR16, indicated the presence of 32, 61 and 64 copies, respectively. Cluster analysis based on hybridization patterns grouped together moderately virulent isolates Ni1, Ni7 and Ni8, thus suggesting a positive correlation between virulence typing and cluster analysis based on molecular data. Variability analysis of N. indica isolates will aid in checking new resistant sources in host germplasm
External Databases
http://link.springer.de/link/service/journals/00122/bibs/0100003/01000569.htm
Keyword
cluster analysis
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-100-569
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||