Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-100-1100

[Submit comment/correction]

Reference	TAG-100-1100
Title	A leucine to proline mutation in puroindoline b is frequently present in hard wheats from Northern Europe
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	1100-1107
Author	Lillemo M Morris CF
Abstract	Summary: Endosperm hardness in wheat (Triticum aestivum L.) is determined by one major genetic factor, the Hardness (Ha) gene on the short arm of chromosome 5D. Grain hardness has previously been reported to result from either a failure to express puroindoline a (Pina-D1b) or a glycine to serine mutation at position 46 in puroindoline b (Pinb-D1b). In this study, which involves a large survey of 343 wheat genotypes of mostly Northern European origin, we report two new mutations in puroindoline b associated with hard endosperm. These were characterized as involving a leucine to proline change at position 60 (Pinb-D1c), and a tryptophan to arginine change at position 44 (Pinb-D1d), respectively. While the former seems to be widely distributed in germplasm around the world, the latter was only found in three winter wheats from Sweden and Netherlands. As discussed in the paper, the three known mutations in puroindoline b can be considered 'loss-of-function' mutations (i.e. soft to hard), and structural analysis may serve to predict that their dramatic effect on wheat grain texture is a result of reduced lipid-binding ability
External Databases	https://doi.org/10.1007/s001220051392
Allele	Pina-D1a (Triticum) Pinb-D1a (Triticum) Pinb-D1c (Triticum) Pinb-D1d (Triticum)
Keyword	animal [ Show all 34 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.