GrainGenes Reference Report: TAG-106-1059
Reference
TAG-106-1059
Title
RAPD polymorphisms in spring wheat cultivars and lines with different level of Fusarium resistance
Journal
Theoretical and Applied Genetics
Year
2003
Volume
106
Pages
1059-1067
Author
Sun G Bond M Nass H Martin R Dong Z
Abstract
Summary: Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 35 spring wheat cultivars and lines with different levels of Fusarium resistance. The objectives of this study were to determine RAPD-based genetic similarity between accessions and to derive associations between Fusarium head blight (FHB) and RAPD markers. Two bulked DNA from either highly resistant lines or susceptible lines were used to screen polymorphic primers. Out of 160 screened primers, 17 primers generated reproducible and polymorphic fragments. Genetic similarity calculated from the RAPD data ranged from 0.64 to 0.98. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm, which corresponded well with the results of principal component analysis and separated the 35 genotypes into two groups. Association analysis between RAPD markers and the FHB index detected three RAPD markers, H191000, F2500 and B12400, significantly associated with FHB-resistant genotypes. These results suggest that a collection of unrelated genotypes can be used to identify markers linked to an agronomically important quantitative trait like FHB. These markers will be useful for marker-assistant breeding and cloning
Keyword
accessions
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-106-1059
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||