GrainGenes Reference Report: TAG-113-429
Reference
TAG-113-429
Title
Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat.
Journal
Theoretical and Applied Genetics
Year
2006
Volume
113
Pages
429
Author
Yu J Herrmann M
Abstract
The powdery mildew resistance from Avena macrostachya was successfully introgressed into hexaploid oat (A. sativa). Genetic analysis of F1, F2, F3 and BC1 populations from two powdery-mildew resistant introgression lines revealed that the resistance is controlled by a dominant gene, tentatively designated Eg-5. Molecular marker analysis was conducted using bulked-segregant analysis in two segregating F3 populations. One codominant simple sequence repeats (SSR) marker AM102 and four AFLP-derived PCR-based markers were successfully developed. The SSR marker AM102 and the STS marker ASE41M56 were linked to the gene Eg-5, with genetic distances of 2 and 0.4 cM, respectively, in both mapping populations. Three STS markers (ASE45M56, ASE41M61, ASE36M55) co-segregated with Eg-5 in one population while two (ASE45M56, ASE36M55) of them linked to Eg-5 with a genetic distance of 1 cM in another population. The gene was further mapped to be in a region corresponding to linkage group 22_44+18 in the Kanota × Ogle (KO) hexaploid oat map by comparative mapping. To our knowledge, this is the first report of mapping powdery-mildew resistance in hexaploid oat. The new resistance source of A. macrostachya, together with the tightly linked markers identified here, could be beneficial in oat breeding programmes.
External Databases
https://doi.org/10.1007/s00122-006-0308-0
Gene
Eg-5 (Avena)
Germplasm
Am28 (oat) Flamingsprofi (oat) Am27 (oat) Neklan (oat)
Locus
ASE41M61 ASE41M56 ASE45M56 ASE36M55 Eg-5
Map Data
Oat-2006-Am28xF Oat-2006-Am27xN
Probe
ASE41M56 ASE45M56 ASE36M55
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-113-429
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||