GrainGenes Reference Report: TAG-119-1281
Reference
TAG-119-1281
Title
Molecular mapping of leaf rust resistance gene Rph14 in Hordeum vulgare.
Journal
Theoretical and Applied Genetics
Year
2009
Volume
119
Pages
1281-1288
Author
Golegaonkar PG Karaoglu H Park RF
Abstract
An incompletely dominant gene conferring resistance to Puccinia hordei, Rph14, identified previously in an accession of Hordeum vulgare, confers resistance to all known pathotypes of P. hordei in Australia. Knowledge of the chromosomal location of Rph14 and the identification of DNA markers closely linked to it will facilitate combining it with other important leaf rust resistance genes to achieve long lasting resistance. The inheritance of Rph14 was confirmed using 146 and 106 F(3) lines derived from the crosses 'Baudin'/'PI 584760' (Rph14) and 'Ricardo'/'PI 584760' (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks using DArT markers located Rph14 to the short arm of chromosome 2H. DArT marker bPb-1664 was identified as having the closest genetic association with Rph14. PCR based marker analysis identified a single SSR marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cm in the 'Baudin'/'PI 584760'and 'Ricardo'/'PI 584760' populations, respectively.
External Databases
http://dx.doi.org/10.1007/s00122-009-1132-0
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-119-1281
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||