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GrainGenes Reference Report: BIU-230-255

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Reference	BIU-230-255
Title	The analysis of genetic diversity of components of rye hybrids
Journal	Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin
Year	2003
Volume	230
Pages	255-263
Author	Rafalski A Gawel M Wisniewska I
Language	Polish
Abstract	The genetic diversity among 26 maternal single crosses, 8 restorers, 17 male sterile inbred lines and 13 nonrestorer inbred lines was evaluated using PCR marker analysis. These materials were the components of rye hybrids investigated in the pre-official trials at the Plant Breeding and Acclimatization Institute, Danko Breeding Co. Ltd. and Poznan Breeding Co. Ltd in Poland during 2001/02. For the evaluation of genetic diversity of rye breeding materials, the semi-specific PCR system using primers targeting intron-exon sequences of plant genes was applied. The evaluation of genetic diversity among maternal hybrids and restorers was based on analysis of 317 DNA fragments, and the genetic distances among inbred lines were calculated from analysis of 370 DNA fragments. Both the estimated data of genetic distances of materials and the cluster analysis based on PCR data indicated that hybrid components from different breeding centres exhibited somewhat different genetic characters
Keyword	acclimatization [ Show all 17 ]

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