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GrainGenes Reference Report: BIU-230-265

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Reference	BIU-230-265
Title	The chromosomal location of markers of aluminium tolerance in rye
Journal	Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin
Year	2003
Volume	230
Pages	265-274
Author	Wisniewska I Rafalski A
Language	Polish
Abstract	DNA hybridization of the set of wheat-rye addition lines is the technique commonly used for chromosomal location of DNA based markers in rye. The other approach is the DNA hybridization of available set of substitution lines of triticale. Because of the tendency for spontaneous loss of alien chromosomes in wheat-rye addition lines, their presence was verified by Southern dot blot hybridization using two DNA probes characteristic of rye specific dispersed repetitive sequence. Both probes revealed identical or similar hybridization signals during the analysis of DNA obtained from 103 single seedlings of wheat-rye addition lines. These results confirmed the total or partial loss of rye DNA in some seedlings. The DNA of single seedlings which showed strong and uniform hybridization signal was selected for chromosomal location of rye fragments. The DNA of the set of D/R substitution lines of triticale was also included in these experiments. Four of 18 fragments used as the probes showed increase of hybridization signal to DNA of 4R addition line of wheat with simultaneous decrease of the signal to DNA of 4R/4D substitution line of triticale. The hybridization pattern of the remaining fragments was not informative because of similar intensity of signals to all additions and substitutions or of not consistent differences in intensity of signals to some additions and substitutions
Keyword	addition line [ Show all 14 ]

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