GrainGenes Reference Report: BIU-231-237
Reference
BIU-231-237
Title
Usefulness of ISSR markers for identification of inbred lines and mapping of rye genome
Journal
Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin
Year
2004
Volume
231
Pages
237-246
Author
Stojaowski S Milczarski P Masojc P
Language
Polish
Abstract
A study was conducted to evaluate the utility of ISSR markers as a tool for the identification of inbred lines and for the construction of genetic map of rye. Sixteen rye inbred lines and a mapping population composed of 94 individual F SUB 2 plants from the 544 x Ot0-20 intercross were used. Twelve primers, which generated strong and reproducible amplification products, were selected out of 57 primers tested. The selected primers revealed 29 polymorphic bands which allowed the identification of lines and the estimation of their genetic diversity. A dendrogram developed on the basis of genetic similarities proved to be generally consistent with the pedigree data. Out of eight markers used for the analysis of F SUB 2 intercross population, six showed segregation consistent with 3:1 expectation for a single locus. The presented results of ISSR marker segregation suggest that this PCR-based method should be suitable for the construction of rye genetic map. However, its efficiency seems to be comparable with that of RAPD
Keyword
cross
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: BIU-231-237
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||