GrainGenes Reference Report: MCB-17-4622
Reference
MCB-17-4622
Title
A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans
Journal
Molecular and Cellular Biology
Year
1997
Volume
17
Pages
4622-4632
Remark
Discusses transcription factor / regulator : MED6
Author
Lee YC Min S Gim BS Kim YJ
Abstract
A temperature-sensitive mutation was obtained in Med6p, a component of the mediator complex from the yeast Saccharomyces cerevisiae. The mediator complex has been shown to enable transcriptional activation in vitro. This mutation in Med6p abolished activation of transcription from four of five inducible promoters tested in vivo. There was no effect, however, on uninduced transcription, transcription of constitutively expressed genes, or transcription by RNA polymerases I and III. Mediator-RNA polymerase II complex isolated from the mutant yeast strain was temperature sensitive for transcriptional activation in a reconstituted in vitro system due to a defect in initiation complex formation. A database search revealed the existence of MED6-related genes in humans and Caenorhabditis elegans, suggesting that the role of mediator in transcriptional activation is conserved throughout the evolution.
External Databases
https://www.ncbi.nlm.nih.gov/pubmed/9234719
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: MCB-17-4622
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||