GrainGenes Reference Report: CPP-26-156
Reference
CPP-26-156
Title
Mapping of a covered smut resistance gene in barley (Hordeum vulgare)
Journal
Canadian Journal of Plant Pathology
Year
2004
Volume
26
Pages
156-166
Author
Grewal TS Rossnagel BG Scoles GJ
Abstract
Covered smut of barley, caused by Ustilago hordei (U. segetum), is a seed-borne disease. Economic loss is due to decreased yield and to contamination of healthy grains with black teliospores. Molecular marker-assisted selection for covered smut is desirable, because traditional screening in the greenhouse and field is time consuming, expensive, and unreliable. The randomly amplified polymorphic DNA (RAPD) marker OPJ10450, tightly linked to covered smut resistance in the barley line Q21861, was mapped to the short arm of chromosome 5 (1H) near the restriction fragment length polymorphic marker Act8A in the 'Harrington'/TR306 mapping population. The covered smut resistance gene in Q21861 was given the provisional designation of Ruhq. Sequence tagged site marker aHor2 and RAPD marker OPO6780 on the short arm of chromosome 5 (1H) were closely linked to Ruhq. The amplified fragment length polymorphic marker E-AAA/M-CCC265 was also linked to Ruhq. These markers will be useful for marker-assisted selection for covered smut resistance in barley
Keyword
amplified polymorphic dna
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CPP-26-156
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||