Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: CHR-103-331

[Submit comment/correction]

Reference	CHR-103-331
Title	Physical mapping of 5S rDNA reveals a new locus on 3R and unexpected complexity in a rye translocation used in chromosome mapping
Journal	Chromosoma
Year	1994
Volume	103
Pages	331-337
Author	Alonso Blanco [ Show all 6 ]
Abstract	Using fluorescence in situ hybridization (FISH) with probe pScT7, three different 5S rDNA loci were detected in the satellite of rye chromosome lR (5SDna-R1) and in the short arms of chromosomes 3R (5SDna-R3) and 5R (5SDna-R2) respectively All three loci showed polymorphism for the hybridization signal intensity In order to determine the localization of these rye 5S rDNA multigene loci with higher precision within the corresponding chromosome arms the probe pScT7 was physically mapped by FISH in relation to the following five translocations (Wageningen Tester Set): T850W (1RS/4RL), T248W (1RS/6RS), T273W 1RS/5RL), T305W (2RS/5RS) and T240W (3RS/5RL) accurate physical maps of the translocation breakpoints had previously been made using electron microscope analysis of spread pachytene synaptonemal complexes of heterozygotes for the different translocations The results indicate that locus 5SDna-R3 is located between the breakpoint of translocation T240W and the telomere, where locus 5SDNA-R2 is located between the breakpoint of translocation T305W and the centromere, the hybridization of probe pScT7 on T305W translocated chromosomes demonstrating the complex nature of this translocation On the other hand, the simultaneous detection of probes pScT7 and pTA71 (18S-5.8S-26S rDNA) with two different fluorochromes, indicated that the breakpoints of translocations T850W and T248W are located between loci Nor-RI and 5SDna-R1.
Keyword	chromosome [ Show all 9 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.