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GrainGenes Reference Report: BRS-54-409

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Reference	BRS-54-409
Title	Spontaneous brassinolide-insensitive barley mutants ' uzu ' adapted to East Asia
Journal	Breeding Science
Year	2004
Volume	54
Pages	409-416
Author	Saisho D [ Show all 6 ]
Abstract	The uzu gene shows typical semi-dwarf plant type and has resulted in lodging resistance and improved canopy structure in barley. The geographic distribution of uzu lines is limited in East Asia including Japan, Korean peninsula and China. For many years, the majority of cultivated 6-rowed barley cultivars in southern Japan were of the uzu type. The inheritance of the uzu gene, located on chromosome 3H, is monogenic recessive and its expression is pleiotropic during the developmental process. In cereal crop plants, several semi-dwarf genes have been widely used in breeding programmes to increase productivity and it is known that several of these semi-dwarf genes were derived from gibberellin (GA)-related mutations. The barley dwarfing gene uzu is independent to GA. In this study, we characterized the molecular aspects of the uzu gene. As the result of a comprehensive study using several pairs of isogenic lines, we found the morphological and physiological similarities of barley uzu gene to the rice dwarf mutant, d61 . D61 encodes OsBRI1 , which is a homologous gene for Brassinosteroid insensitive 1 in Arabidopsis thaliana . We also found that uzu lines specific single nucleotide polymorphism (SNP) contributed for amino acid substitution in the barley homologous sequence OsBRI1 , HvBRI1 . Linkage analysis using a segregating population for uzu showed the co-segregation between uzu and HvBRI1 . Furthermore, as the results of derived cleaved amplified polymorphic sequences (dCAPS) marker analysis using more than 260 uzu landraces and the genomic sequencing analysis of HvBRI1 gene derived from 19 barley accessions, all the uzu lines studied had the same SNP in the putative kinase domain of HvBRI1 . Based on these findings, we discussed the phylogeny of uzu landraces in East Asia
Keyword	arabidopsis thaliana [ Show all 28 ]

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