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GrainGenes Reference Report: BOS-46-867

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Reference
BOS-46-867
Title
Identification and microsatellite markers of a resistance gene to powdery mildew in common wheat introgressed from Triticum durum
Journal
Acta Botanica Sinica
Year
2004
Volume
46
Pages
867-872
Author
Zhu Z-D
Kong X-Y
Zhou R-H
Jia J-Z
Abstract
A powdery mildew resistance gene in a BC3F2 population, derived from a cross made between an amphidiploid of Triticum durum Desf.-Aegilops caudata L. and T aestivum L. cv. Laizhou 953, was identified. Genetic analysis of resistance to powdery mildew in BC3F2 population and derived BC3F3 families indicated a single dominant gene controlled the resistance. By bulk segregation analysis, two microsatellite markers, Xgwm3ll and Xgwm382, were identified to be closely linked to the resistance gene with genetic distance of 5.9 cM and 4.9 cM, respectively. DNA from T. durum accession DR147, Ae. caudata acc. Ael4, and recurrent parent wheat (Triticum aestivum L.) cv. Laizhou 953 were amplified with primer pairs WMS311 and WMS382, the specific bands related to the resistance gene were only present in T. durum acc. DR147. Results showed that the resistance gene originated in T. durum acc. DR147. Based on the location of the linked microsatellite markers, the resistance gene was located on the telomeric region of chromosome 2AL in wheat. Temporarily, the resistance gene was designated as PmDR147. The relation of this gene and Pm4 was discussed.
Gene
PmDR147
Germplasm
DR147
Ae14
Laizhou953
Locus
PmDR147
QTL
QPm.DR147xL953-2A
QTL2098_2A-Zhu_et_al._2004
Trait Study
Powdery mildew, DR147/Ae14 x Laizhou953, Zhu2004

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