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GrainGenes Reference Report: JCS-41-1

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Reference	JCS-41-1
Title	Identification of new puroindoline genotypes and their relationship to flour texture among wheat cultivars
Journal	Journal of Cereal Science
Year	2005
Volume	41
Pages	1-6
Author	Ikeda TM [ Show all 6 ]
Abstract	Puroindoline genotypes (Pina and Pinb) and their encoded proteins related to grain hardness were studied in various common wheat cultivars from Australia, China, Japan, Korea and North America. Most of the hard wheats had the Pinb-D1b genotype with a glycine to serine mutation at position 46. In addition to the known Pina and Pinb genotypes, cultivars were found with Pina and Pinb double-null mutations (Pina-D1b/Pinb-D1h (t)) and a new Pinb frameshift mutation (designated Pinb-D1i (t)) within the region encoding a tryptophan-rich domain. This new Pinb frameshift mutation was found only in Chinese cultivars. Endosperm proteins encoded by Pina and Pinb in these cultivars were analysed by 2D-gel electrophoresis (IPGxSDS-PAGE). Cultivars with Pina and Pinb double-null mutations showed no PIN-a or PIN-b protein, and cultivars with Pinb-D1i (t) had no PIN-b protein. Surprisingly, cultivars with Pinb-D1b had severely reduced amounts of PIN-b and cultivars with Pinb-D1c showed no PIN-b proteins. Grain hardness among cultivars having mutated Pinb may be explained by the amount of PIN-b protein and not by the type of amino acid substitutions.
External Databases	https://doi.org/10.1016/j.jcs.2004.10.002
Allele	Pina-D1k (Triticum) Pinb-D1p (Triticum) Pinb-D1v (Triticum) Pinb-D1y (Triticum)
Gene	Pina-D1 (Triticum)

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