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GrainGenes Reference Report: JBR-55-187

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Reference	JBR-55-187
Title	A tryptophan-to-arginine change in the tryptophan-rich domain of puroindoline b in five French bread wheat cultivars.
Journal	Journal of Genetics and Breeding
Year	2001
Volume	55
Pages	187-189
Author	Corona V Gazza L Zanier R Pogna NE
Abstract	Puroindolines, the tryptophan-rich proteins controlling grain hardness in wheat, appeared as two pairs of 13 kDa polypeptides in the Acid-PAGE (A-PAGE) and two-dimensional A-PAGE x SDS-PAGE patterns of starch-granule proteins from wild allotetraploid wheat Aegilops ventricosa Tausch. (2n = 4x = 28, genomes DvDvNvNv). Puroindoline pair a1 + a2 reacted strongly with an antiserum specific for puroindoline-a from common wheat (Triticum aestivum L.), whereas puroindoline pair b1 + b2 exhibited A-PAGE relative mobilities similar to that of puroindoline-b in Aegilops tauschii (Coss.), the D-genome donor to both common wheat and Ae. ventricosa. Puroindolines a2 and b1 were found to be encoded by alleles Pina-D1a and Pinb-D1h on chromosome 5Dv, respectively, whereas puroindolines a1 and b2 were assumed to be under the genetic control of chromosome 5Nv. Puroindoline a1 encoded by the novel Pina-N1a allele exhibited a high level of amino acid variation with respect to puroindoline-a. On the other hand, the tryptophan-rich region of puroindoline b2 encoded by allele Pinb-N1a showed a sequence change from lysine-42 to arginine, with no effect on the amount of protein b2 accumulated on the starch granules. A partial duplication of the pin-B gene (Pinb-relic) was identified about 1100 bp downstream from Pinb-D1 on chromosome 5Dv. The present findings are the first evidence of a tetraploid wheat species in which four puroindoline genes are expressed. The potential of Ae. ventricosa as a source of genes that may be used to modulate endosperm texture and other valuable traits in cultivated wheat species is discussed.
External Databases	https://doi.org/10.1007/s11032-005-4886-3
Allele	Pinb-D1d (Triticum)

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