GrainGenes Reference Report: CCM-69-659
Reference
CCM-69-659
Title
A room-temperature, rapid method for the determination of protein in wheat and other grains by the biuret reaction
Journal
Cereal Chemistry
Year
1992
Volume
69
Pages
659-664
Author
Strong F, III Duarte A
Abstract
A rapid, room-temperature, biuret method for determining protein in grains was developed Samples of wheat ground to pass a 0.105-mm screen were mixed for 5 min in a blender with 2 ml of isopropanol, 700 mg of basic cupric carbonate (bright green), and 100 ml of 0.500 M NaOH in 30% isopropanol After filtering with suction through a glass fiber filter, absorbance was measured at 550 nm A calibration curve for wheat of absorbance vs protein concentration gave a slope of 0.350 cm-1 g-1 L-1, compared to 0.310 for the official AACC method using heat. Satisfactory results were also obtained with rice and soybeans.
Keyword
biuret protein content rapid-methods temperature wheat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CCM-69-659
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||