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GrainGenes Reference Report: CRS-35-1144

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Reference	CRS-35-1144
Title	Agronomic performance of barley lines derived from tissue culture
Journal	Crop Science
Year	1995
Volume	35
Pages	1144-1148
Author	Bregitzer P Poulson M
Abstract	The practical application of in vitro genetic manipulations to plant breeding may be hindered by unselected and potentially undesirable somaclonal variation To determine the potential for recovering cultivars from tissue cultures without somaclonal variation, 30 families of tissue culture derived lines from six barley (Hordeum vulgare L.) cultivars were analyzed for agronomic performance Each family was derived from a single regenerated plant that in turn was derived from an immature embryo callus culture Four to six R2 plants were selected from each family based on phenotypic similarity to the uncultured parental controls One-hundred-seventy-four R2 derived lines were advanced by bulking to the R5 generation The results of replicated field trials indicated that the agronomic performance for the majority of these lines was altered by passage through tissue culture and that the alterations were generally undesirable Cultivar-dependent differences were noted in the frequency and degree of agronomic alterations. Families within cultivars was an important source of variation, but selections within families was not. Successful selection of tissue culture derived barley lines without somoclonal variation therefore may be cultivar dependent and may require screening large populations of regenerated plants.
Keyword	agronomic-characteristics [ Show all 9 ]

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