Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: CRS-42-1812

[Submit comment/correction]

Reference	CRS-42-1812
Title	Substitutions and deletions of genes related to grain hardness in wheat and their effect on grain texture
Journal	Crop Science
Year	2002
Volume	42
Pages	1812-1817
Author	Tranquilli G Heaton J Chicaiza O Dubcovsky J
Abstract	Summary: The Hardness (Ha) locus on chromosome 5D is the main determinant of grain texture in hexaploid wheat (Triticum aestivum L.). Puroindoline (Pina-D1, Pinb-D1) and Grain Softness Protein (Gsp-D1) genes are tightly linked at this locus. Additional copies of the Gsp-1 gene are present on chromosomes 5A and 5B. Mutations in the Pina-D1 and Pinb-D1 genes have been individually associated with grain hardness, but it is not known if mutations at both loci may further increase hardness or if additional copies may reduce it. In addition, there is no clear evidence of the effect of the Gsp-1 genes on grain texture. To answer these questions, we compared the effect of different dosages of puroindoline and Gsp-1 genes on grain texture. Isogenic substitution and deletion lines for homoeologous group 5 in 'Chinese Spring' (CS) were evaluated in two replicated field trials with 13 blocks each. Deletions or allelic variants of Gsp-A1 and Gsp-B1 did not produce significant effects on grain texture, suggesting that these genes do not have a critical role in grain hardness. Simultaneous deletions of Pina-D1 and Pinb-D1 in deletion line 5DS-2 and substitution line CS (Red Egyptian 5D) resulted in significantly higher hardness index values than all other lines including CS (Timstein 5D) carrying a single Pina-D1 deletion (P = 0.02). The incorporation of additional copies of Pina-A(m)1 and Pinb-A(m)1 from T. monococcum L. in recombinant substitution line 5A/5A(m) in CS resulted in significantly softer grains than those from the CS control (P < 0.01)
External Databases	https://doi.org/10.2135/cropsci2002.1812
Allele	Pina-D1k (Triticum) Pinb-D1q (Triticum) Gsp-D1a (Triticum) Gsp-D1j (Triticum)
Gene	Pina-D1 (Triticum)
Germplasm	CS*/Red Egyptian 5D substitution line
Keyword	allelic [ Show all 27 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.