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GrainGenes Reference Report: CRS-45-1858

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Reference	CRS-45-1858
Title	Phospholipid Acyl Chain and Phospholipase Dynamics during Cold Acclimation of Winter Wheat
Journal	Crop Science
Year	2005
Volume	45
Pages	1858-1867
Author	Skinner DZ [ Show all 6 ]
Abstract	Phospholipid (PL) composition is known to change in plants exposed to cold temperature. The dynamics of PL acyl chain pairs and genes encoding phospholipase enzymes were studied in winter wheat (Triticum aestivum L.) during cold acclimation. Mass spectrometry was used to characterize PL dynamics, and quantitative real-time PCR was used to characterize phospholipase gene mRNA transcript dynamics during cold acclimation. The proportion of PLs with mismatched acyl chains decreased concomitantly with an increase in total PLs during the first week of cold exposure. Proportions of mismatched acyl chains then increased, while total PLs varied little. Numbers of mRNA transcripts of phospholipase (PL)D, PLC, and PLA2 increased in response to cold and remained at elevated levels throughout a 4-wk period. Lysophosphatidylcholine (LPC) increased as much as 14-fold over the 5-wk period and increased significantly less in a less cold tolerant cultivar than more tolerant cultivars. It appeared that newly synthesized PLs with equal-length acyl chains form a part of the initial response to cold temperature; they are then modified to contain near-initial levels of mismatched acyl chains during acclimation. LPC is a highly active signal molecule and PLA2, PLC, and PLD are involved in generation of phospholipid-based signaling molecules; hence, it appeared PL signaling is involved in initial and continuing responses to cold temperature.
External Databases	http://crop.scijournals.org/cgi/content/abstract/45/5/1858

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