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GrainGenes Reference Report: CUG-24-533

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Reference	CUG-24-533
Title	The nad6 gene and the exon d of nad1 are co-transcribed in wheat mitochondria
Journal	Current Genetics
Year	1993
Volume	24
Pages	533-538
Author	Haouazine N Takvorian A Jubier M Michel F Lejeune B
Abstract	The exon d of nad1 is located 993 bp upstream of the nad6 gene in the wheat mitochondrial genome Transcription analyses of both sequences (nad1 exon d and the nad6 gene) were done by Northern hybridization using RNA from wheat seedlings and tissue cultures derived from immature embryos A complicated pattern was generated with a probe including exon d of nad1 and the whole nad6 gene An 0.71-kb transcript is specific to nad1 exon d whereas a 1.2-kb transcript is specific to the nad6 gene Three larger transcripts hybridize to both probes suggesting that nad1 exon d and nad6 are co-transcribed. This co-transcription has been directly demonstrated by cDNA synthesis on mtRNAs and sequencing of the PCR amplification product.
Keyword	callus [ Show all 9 ]

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