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GrainGenes Reference Report: EJB-223-917

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Reference	EJB-223-917
Title	Cloning of a wheat 15 kDa grain softness protein (GSP). GSP is a mixture of puroindoline like polypeptides
Journal	European Journal of Biochemistry
Year	1994
Volume	223
Pages	917-925
Author	Rahman S Jolly CJ Skerritt JH Wallosheck A
Abstract	The wheat starch 15-kDa protein (called grain softness protein or GSP) consists of a major polypeptide and several minor polypeptides. An antiserum raised against GSP was used to screen a wheat cDNA library. A cDNA family encoding approximately 15-kDa proteins that included a heptapeptide sequence previously isolated from protease digests of GSP was identified. A partial cDNA was used in a prokaryotic expression system to produce a fusion protein which reacted strongly against the original anti-GSP serum. A new antiserum raised against the fusion protein produced a weak reaction against a 15-kDa polypeptide extracted from wheat seeds. The results suggest that the proteins encoded by the cDNA family form a minor component of the mixture of 15-kDa polypeptides defined as GSP. RNA complementary to the cDNAs could be extracted from both soft and hard wheat grains from about half-way through grain filling. The encoded proteins are novel members of the 2S superfamily of seed proteins, a diverse family of proteins which maintain a characteristic framework of cysteine residues. The deduced proteins show the highest similarity to the oat 16-kDa avenin and to wheat puroindoline (a lipid-binding 15-kDa protein from wheat). Review of previously published data shows that puroindoline is also closely related to the major polypeptide of GSP, suggesting that the lipid-binding properties of GSP polypeptides may influence grain softness.
External Databases	https://doi.org/10.1111/j.1432-1033.1994.tb19069.x
Allele	Gsp-D1a (Triticum)
Gene	Gsp-1 (Triticum) Gsp-A1 (Triticum) Gsp-B1 (Triticum)
Probe	pGsp

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