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GrainGenes Reference Report: FBS-579-6890

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Reference
FBS-579-6890
Title
Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars
Journal
FEBS Letters
Year
2005
Volume
579
Pages
6890-6894
Author
Wherrett T
Shabala S
Pottosin I
Abstract
Patch-clamp experiments revealed that near isogenic ET8 (A1-tolerant) and ES8 (A1-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 mu M vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pretreatment. However, the activation threshold was reduced in A1-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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