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GrainGenes Reference Report: FBS-582-3922

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Reference	FBS-582-3922
Title	Point mutations in the barley HvHAK1 potassium transporter lead to improved K(+)-nutrition and enhanced resistance to salt stress.
Journal	FEBS Letters
Year	2008
Volume	582
Pages	3922-3928
Author	Mangano S Silberstein S Santa-Mara GE
Abstract	Members of group I KT-HAK-KUP transporters play an important role in K(+) acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K(+)-capture, and improved NaCl, LiCl and NH(4)Cl tolerance, to yeast cells. Improved K(+)-capture was linked to an enhanced V(max). Results reveal an intrinsic protective effect of K(+), and assign an important role to the 8th transmembrane domain, as well as the C-terminus, in determining the maximum capacity for the transport of K(+) in KT-HAK-KUP transporters.
External Databases	http://dx.doi.org/10.1016/j.febslet.2008.10.036

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