GrainGenes Reference Report: GES-36-379
Reference
GES-36-379
Title
Cytogenetic and molecular identification of three Triticum aestivum-Leymus racemosus translocation addition lines.
Journal
Journal of Genetics and Genomics (Yi Chuan Xue Bao)
Year
2009
Volume
36
Pages
379-385
Author
Wang L Yuan J Bie T Zhou B Chen P
Abstract
Chromosome 2C from Aegilops cylindrica has the ability to induce chromosome breakage in common wheat (Tritivum aestivum). In the BC(1)F(3) generation of the T. aestivum cv. Chinese Spring and a hybrid between T. aestivum-Leymus racemosus Lr.7 addition line and T. aestivum-Ae. cylindrica 2C addition line, three disomic translocation addition lines (2n = 44) were selected by mitotic chromosome C-banding and genomic in situ hybridization. We further characterized these T. aestivum-L. racemosus translocation addition lines, NAU636, NAU637 and NAU638, by chromosome C-banding, in situ hybridization using the A- and D-genome-specific bacterial artificial chromosome (BAC) clones 676D4 and 9M13; plasmids pAs1 and pSc119.2, and 45S rDNA; as well as genomic DNA of L. racemosus as probes, in combination with double ditelosomic test cross and SSR marker analysis. The translocation chromosomes were designated as T3AS-Lr7S, T6BS-Lr7S, and T5DS-Lr7L. The translocation line T3AS-Lr7S was highly resistant to Fusarium head blight and will be useful germplasm for resistance breeding.
External Databases
http://dx.doi.org/10.1016/S1673-8527(08)60127-7
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GES-36-379
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||