GrainGenes Reference Report: GNM-37-460
Reference
GNM-37-460
Title
Recombination of alleles conferring specific resistance to powdery mildew at the Mla locus in barley.
Journal
Genome
Year
1994
Volume
37
Pages
460-468
Author
Mahadevappa M DeScenzo RD Wise RP
Type
Article
Abstract
In barley (Hordeum vulgare L.), the Mla locus conditions reaction to the powdery mildew fungus, Erysiphe graminis f. sp. hordei. Enrichment for genetic recombinants in the Mla region is possible by screening for recombination events between the flanking endosperm storage proteins, hordeins C and B. Reciprocal crosses were made between the Franger (C.I. 16151) and Rupee (C.I. 16155) lines carrying the [Mla6 + Mla14] and Mla13 alleles, respectively. Recombinants were identified from F2 segregants by analyzing the extracted hordein polypeptides by SDS-polyacrylamide gel electrophoresis. Two hundred seventy six recombinant gametes were identified from the 1800 seeds that were screened. Recombination of Mla alleles was analyzed by inoculating F4 recombinant lines with three isolates of E. graminis; A27, 5874, and CR3, which recognize specific Mla alleles. The linkage order established is Hor1, Mla6, Mla13, Mla14, and Hor2. The genetic distances between Hor1 - Mla6, Mla6 - Mla13, and Mla13 - Hor2, obtained using Mapmaker 3.0b F3 intercross analysis, are 3.9, 0.2, and 5.2 cM respectively.
Keyword
barley hordein Mla powdery mildew recombination
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GNM-37-460
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||