Query (optional)   in Class  

GrainGenes Reference Report: GNM-44-936

[Submit comment/correction]

Reference
GNM-44-936
Title
Low responsiveness of six-rowed genotypes to androgenesis in barley does not have a pleiotropic basis
Journal
Genome
Year
2001
Volume
44
Pages
936-940
Author
Castillo AM
Cistue L
Romagosa I
Valles M
Valles MP
Abstract
A heterozygous mutant for the two- and six-rowed character was isolated in the barley cultivar Igri through application of sodium azide to isolated microspore cultures and posterior regeneration. Six-rowed and two-rowed homozygotic plants were subsequently identified in the self-pollinated M2 progenies of the original heterozygous M1. Detailed molecular markers confirmed the isogenic nature of this recovered mutant and the original cultivar Igri. A comparative study of the anther culture response of this six-rowed induced mutant vs. diploid 'Igri' was performed to assess whether the two- or six-rowed gene influences anther culture response in barley through a pleiotropic effect or via linkage disequilibrium. No significant differences for any of the recorded variables throughout the in vitro regeneration process were detected between the 'Igri' six-rowed mutant and any of their two-rowed isogenic lines. This suggests that row-type association with anther culture response in barley cultivars is due to the effect of a tight linkage with other genes directly responsible for androgenic response
Keyword
androgenesis
[ Show all 48 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.