GrainGenes Reference Report: JCS-18-197
Reference
JCS-18-197
Title
New data supporting high Mr glutenin subunit 5 as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12
Journal
Journal of Cell Science
Year
1993
Volume
18
Pages
197-205
Author
Lafiandra D D'Ovidio R Porceddu E Margiotta B Colaprico G
Abstract
One- and two-dimensional electrophoretic separations were performed on high M(r) subunits of glutenin of the bread wheat cultivar Fiorello, which was reported to possess the recombinant 5 + 12 subunits at the Glu-D1 locus Comparison with results for the bread wheat cultivar Cheyenne, possessing the pair 5 + 10, indicated that subunit 5 of Fiorello was different, both in size and isoelectric point, from subunit 5 of Cheyenne RP-HPLC separations of high M(r) glutenin subunits also indicated that the two types of subunit 5 were different in surface hydrophobicity The sequence of a cloned DNA fragment isolated from Fiorello, corresponding to the N-terminal part of the protein, indicated the absence of the additional cysteine residue at the beginning of the repetitive domain flanking the N-terminal region typical of subunit 5 The data show that the Dx subunit of Fiorello, indicated as 5*, is more similar to subunit 2 than to subunit 5, raising doubts on previous results excluding subunit 5 as being responsible for the differences in breadmaking quality observed in the pairs 5 + 10/2 + 12
Keyword
baking-quality bread flour glutenin
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JCS-18-197
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||