GrainGenes Reference Report: JCS-30-165
Reference
JCS-30-165
Title
Characterisation of starch from inner and peripheral parts of normal and waxy barley kernels
Journal
Journal of Cell Science
Year
1999
Volume
30
Pages
165-171
Author
Andersson L Fredriksson H Oscarsson-Bergh M Andersson R Aman P
Abstract
A previous microscopical examination of barley kernels indicated an uneven distribution of amylose in the waxy cultivar SW7142-92, where the subaleurone layer was strongly stained by iodine To explain these results, starch was isolated from fractions of inner and peripheral parts of kernels of the waxy cultivar and a cultivar with normal starch Analyses of amylose content showed a higher concentration of amylose in starch from the outer part of the waxy barley kernel, where the amylose, determined by a colorimetric method, made up 8-6% of the starch compared with 5-9% in the middle and 2-2% in the innermost part of the waxy kernel. This difference in amylose distribution was not observed in the normal starch kernel. The results were confirmed by gel permeation chromatography. No differences in amylopectin chain length distribution were detected by size exclusion HPLC and HPAEC-PAD of amylopectins from different parts of the barley kernels.
Keyword
amylopectin
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JCS-30-165
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||