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GrainGenes Reference Report: JEB-44-1067

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Reference	JEB-44-1067
Title	Normalizing development of cultured Triticum aestivum L. embryos. I. Low oxygen tensions and exogenous ABA
Journal	Journal of Experimental Botany
Year	1993
Volume	44
Pages	1067-1073
Author	Hess J Carman J
Abstract	Wheat (Triticum aestivum L. embryos form in dynamically-regulated ovular environments Our objectives were to improve development of cultured immature wheat embryos by simulating, in vitro, abscisic acid (ABA) levels and O2 tensions as found in wheat ovules during zygotic embryogenesis We characterized from intact wheat kernels embryo respiration, embryo morphology and embryo and endosperm +ABA levels at 13, 19 and 25 d post-anthesis (DPA) Young (13 DPA) embryos were then excised and cultured in vitro, where they were exposed to 0.2 or 2.0 mmol m-3 +/- ABA and 2.1, 2.5 or 7.4 mol m-3 (6, 7 and 21%, respectively) gaseous O2. At 6 and 12 d in culture, +ABA levels, embryo respiration and embryo morphology were characterized by treatment. Thirteen-day-old embryos from two different plant populations differed by 17-fold in initial ABA content. However, this difference did not affect precocious germination in vitro, nor did it affect the amount of exogenous ABA required to reduce precocious germination by 40%. In this respect, embryos from both populations were equally sensitive to exogenous ABA. Cavity sap O2 levels (2.1 to 2.5 mol m-3) were much more effective in preventing precocious germination of cultured embryos than were cavity sap levels of ABA (0.2 to 2.0 mmol m-3). The combination of physiological levels of both ABA and O2 largely normalized DW accumulation and embryo morphology without altering endogenous +ABA levels. Residual respiration of cultured embryos was higher than that of embryos grown in situ, and was not influenced by the exogenous O2 and ABA treatments.
Keyword	abscisic acid [ Show all 6 ]

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