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GrainGenes Reference Report: JEB-50-1627

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Reference
JEB-50-1627
Title
Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization
Journal
Journal of Experimental Botany
Year
1999
Volume
50
Pages
1627-1628
Author
Chan YW
Tung WL
Griffith M
Chow KC
Abstract
Summary: A cDNA clone, WRTLP2, encoding an open reading frame of 173 amino acids, was recovered from a cDNA library of winter rye (Secale cereale L. Musketeer). The amino acid sequence deduced from the cloned cDNA exhibits very high sequence similarity (70-95%) with those of extracellular and low molecular weight thaumatin-like proteins of other cereals. It was possible to overexpress this isolated cDNA in Escherichia coli and it was found that the encoded protein of this clone exhibited antifungal activities against several fungal strains
Keyword
amino acid
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