GrainGenes Reference Report: JFC-40-1836
Reference
JFC-40-1836
Title
New quantitative high-performance liquid chromatography method for analysis of gramine in cereal leaf tissue
Journal
Journal of Agricultural and Food Chemistry
Year
1992
Volume
40
Pages
1836-1838
Author
Muir A Westcott N Hinks C
Abstract
Gramine was extracted from fresh and freeze-dried cereal leaf tissue according to a simplified methanol/ammonia extraction procedure and quantified by reversed-phase HPLC without further sample preparation Chromatography was achieved using a modified Pico-Tag solvent system with gradient elution Quantitative recovery (76-78%) of gramine (17-871 micrograms/mL) from plant samples was achieved Recovery of 3-(aminomethyl)indole (AMI),N-methyl-3-(aminomethyl)indole (MAMI), and tryptamine, intermediates in the biosynthesis of gramine, was also demonstrated
Keyword
gramine hordeum vulgare hplc leaves
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JFC-40-1836
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||