Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: JFC-41-1048

[Submit comment/correction]

Reference	JFC-41-1048
Title	Protein isolate from an experimental high-protein wheat and flour
Journal	Journal of Agricultural and Food Chemistry
Year	1993
Volume	41
Pages	1048-1052
Author	Wu Y
Abstract	An experimental high-protein (25% protein, N X 5.7 dry basis) spring wheat (ND 643) and flour from that wheat were used to make protein isolate by alkaline extraction Optimal extraction of protein was at pH 10.7 in 0.03 N sodium hydroxide with a solvent:solid ratio of 6:1 The alkaline extract after centrifugation was adjusted to optimal pH of 6.2 to yield a precipitate and a supernatant. Bran was separated from starch by screening the second alkaline dispersion. The protein contents of the isolates from ground wheat and flour were 90 and 95%, respectively, and accounted for 65 and 68% of the total nitrogen of the starting wheat and flour. Yields of protein isolate were 18 and 16%, respectively, of starting high-protein wheat and flour compared with 10% of starting wheat and flour from a typical hard red winter wheat. Protein isolate had a nitrogen solubility of 96% at pH 2.6 and a minimum solubility of 5.7% at pH 5.8, a hydration capacity of 2.5, an emulsifying activity of 51%, and an emulsion stability of 50%.
Keyword	ph [ Show all 10 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.