GrainGenes Reference Report: JFS-58-1090
Reference
JFS-58-1090
Title
Optimizing assay and extraction of lipoxygenase in wheat germ
Journal
Journal of Food Science
Year
1993
Volume
58
Pages
1090-1094
Author
Bhirud P Sosulski F Sosulski K
Abstract
Using three-level seven-factor response surface methodology, wheat germ lipoxygenase (LPO) assay conditions were standardized The important parameters were concentration of the substrate (linoleic acid), and surfactant (Tween 20), pH and temperature The standardized LPO assay conditions for the 1 mL reaction volume were : 450 micromolars linoleic acid, 129 micromolars Tween 20, 175 mM ethanol, 1 mM EDTA, pH 6.2 (phosphate buffer), ionic strength 100 mM and 40 degrees C LPO extraction conditions were standardized by sequential variation of parameters Optimum conditions were a single extraction of defatted wheat germ flour at 2-5 degrees C with magnetic stirring of an extractant acetate buffer pH 4.5, ionic strength 100 mM, at buffer-to-solid ratio 10:1
Keyword
assays extraction kinetics lipoxygenase wheat germ
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JFS-58-1090
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||