GrainGenes Reference Report: JFS-58-1095
Reference
JFS-58-1095
Title
Thermal inactivation kinetics of wheat germ lipoxygenase
Journal
Journal of Food Science
Year
1993
Volume
58
Pages
1095-1098
Author
Bhirud P Sosulski F
Abstract
Thermal inactivation curves for wheat germ lipoxygenase (LPO) in partially purified and crude extracts were determined in capillary tubes at 60-68 degrees C The biphasic curves fitted a two-fraction first order model suggesting the presence of 2 groups of isozymes At 60 degrees C, the inactivation rate constants were 9112 X 10(-5) sec-1 and 9.174 X 10(-6) sec-1 respectively, for thermolabile phase I and thermostable phase II in the partially purified extract, a difference of one order of magnitude For the temperature change from 60 to 68 degrees C, the rate constants increased by three orders of magnitude, implying a very high sensitivity (for LPO inactivation in partially purified extract delta H = 646261 J.mole-1, delta S = 1619 J.mole-1.K-1 for phase I, delta H = 546099 J.mole-1, delta S = 1298 J.mole-1.K-1 for phase II) to heat by both phases, although phase I was clearly the least stable.
Keyword
heat-treatment inactivation kinetics lipoxygenase wheat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JFS-58-1095
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||