GrainGenes Reference Report: JGV-76-2619
Reference
JGV-76-2619
Title
In vitro transcripts of a full-length cDNA of a naturally deleted RNA2 of barley mild mosaic virus (BaMMV) replicate in BaMMV-infected plants
Journal
The Journal of General Virology
Year
1995
Volume
76
Pages
2619-2623
Author
Timpe U Kuhne T
Abstract
The RNA2 of a German isolate of the bipartite barley mild mosaic bymovirus (BaMMV-ASL1) is 3524 nucleotides long excluding the 3'-terminal poly(A) tail The isolate was propagated by mechanical inoculation for several years Electrophoretic comparison of viral nucleic acids during this period revealed that a spontaneous reduction in the length of the RNA2 occurred resulting in the isolate BaMMV-ASL1a The deleted RNA2 of BaMMV lacked a fragment that was 630 nucleotides long The deletion occurred in the 3' half of the single open reading frame (ORF) found in RNA2; this ORF encodes a polyprotein that has a molecular mass of 98 kDa, which is assumed to be processed autocatalytically into proteins of 25 kDa and 73 kDa. A full-length cDNA of the deleted RNA2 was synthesized and cloned under the control of the phage T7 promoter. In vitro transcripts of the BaMMV-ASL1a clone replicated in barley plants after co-inoculation with a wildtype-like isolate of BaMMV. The deletions in RNA2 of BaMMV-ASL1a and those of a number of other isolates that were examined were found to affect a domain of the putative 73 kDa protein that is obviously not essential for replication but may be important for the transmission of BaMMV by its natural vector Polymyxa graminis.
Keyword
complementary dna
[ Show all 10 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JGV-76-2619
|
| |||
|
| |||
|
| |||
|
| |||
|
| |||
|
| |||
|
| |||
|
| |||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||