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GrainGenes Reference Report: JME-37-273

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Reference
JME-37-273
Title
Chloroplast DNA codon use: evidence for selection at the psb A locus based on tRNA availability
Journal
Journal of Molecular Evolution
Year
1993
Volume
37
Pages
273-280
Author
Morton B
Abstract
Codon use in the three sequenced chloroplast genomes (Marchantia, Oryza, and Nicotiana) is examined The chloroplast has a bias in that codons NNA and NNT are favored over synonymous NNC and NNG codons This appears to be a consequence of an overall high A + T content of the genome This pattern of codon use is not followed by the psb A gene of all three genomes and other psb A sequences examined In this gene, the codon use favors NNC over NNT for twofold degenerate amino acids In each case the only tRNA coded by the genome is complementary to the NNC codon This codon use is similar to the codon use by chloroplast genes examined from Chlamydomonas reinhardtii. Since psb A is the major translation product of the chloroplast, this suggests that selection is acting on the codon use of this gene to adapt codons to tRNA availability, as previously suggested for unicellular organisms.
Keyword
amino acid sequence
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