GrainGenes Reference Report: MGV-2-46
Reference
MGV-2-46
Title
Isolation of barley genome DNA fragment supporting autonomous-plasmid replication in Saccharomycetes
Journal
Molekulyarnaya Genetika Mikrobiologiya i Virusologiya
Year
1989
Volume
2
Pages
46-49
Author
Anan'ev EV Shchipkova NI Khvyrleva TSD Danilevskaya ON
Type
Article
Language
Russian
Abstract
BamH1 fragments of the barley genomic DNA were cloned in Escherichia coli cells on the vector plasmid YIp5 carrying the URA3 gene of Saccharomyces cerevisiae. Yeast cells were transformed by individual plasmid DNA preparations from each clone selected. Approximately 10% of the studied plasmids are able to replicate in yeast cells. Five barley DNA fragments which could support replication of recombinant plasmids in yeast cells belong to moderately repeated DNA and are dispersed through the chromosomes.
Probe
pHv 10 pHv 13-7 pHv 26-20 pHv 67-2 pHv 67-2.9
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: MGV-2-46
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||