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GrainGenes Reference Report: PBR-118-272

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Reference
PBR-118-272
Title
Changes in expression of HMW glutenins of wheat (Triticum aestivum L.) induced by nitrosoethylurea.
Journal
Plant Breeding
Year
1999
Volume
118
Pages
272-274
Author
Svec M
Gregova E
Miklovicova M
Kraic J
Abstract
Summary: The application of a chemical mutagen, N-nitroso-N-ethylurea, to the grains of wheat (Triticum aestivum L.) cv. 'Viginta', provided a mutant line, 'NT-10', with an altered composition of high molecular weight (HMW) glutenin subunits. The qualitative mutation was detected in the Glu-B1 locus by electrophoretic analyses of glutenins. Instead of HMW glutenin subunits 7 + 9 present in the original genotype, a separate HMW subunit 6 was expressed in the mutant line. The other glutenin and gliaden proteins of the mutant line remained unchanged. The mutant line is also characterized by several changes in morphological and physiological characters-stronger stem, wider leaf, bigger spike and higher grain hardness. This is the first communication of the possibility of changing the composition of high molecular weight subunits of wheat glutenin by means of mutagenesis
Keyword
hardness
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