GrainGenes Reference Report: PBR-119-393
Reference
PBR-119-393
Title
Integration of AFLP markers into an RFLP-based map of durum wheat
Journal
Plant Breeding
Year
2000
Volume
119
Pages
393-401
Author
Lotti C Salvi S Pasqualone A Tuberosa R Blanco A
Abstract
Summary: Amplified fragment length polymorphism (AFLP) is a powerful technique which can readily be applied to a wide range of species for mapping purposes. AFLPs were added to a linkage map of durum wheat constructed using restriction fragment length polymorphisms (RFLPs). The mapping population included 65 recombinant inbred lines derived from a cross between the durum wheat cultivar 'Messapia' and accession 'MG4343' of the wild Triticum turgidum ssp. dicoccoides (Korn.). Genomic DNA was digested with MseI (4-cutter) and Sse8387I (8-cutter). Using a silver-staining protocol, 14 primer combinations revealed 421 clearly scorable amplicons including 100 polymorphisms. The presence of nine pairs of bands linked in repulsion phase with each pair generated by one primer combination suggested the presence of codominant alleles; sequence analysis of four band pairs confirmed their codominant nature. The integration of 80 AFLP loci extended the map in several telomeric regions, reduced the size of four large gaps present in the previous map, and eliminated one gap. The new map obtained after the inclusion of the 80 AFLP loci and eight additional RFLP loci spans 2063 cM which represent a 52.6% increment compared with the previous map. Compared with the distribution of RFLPs, no significant clustering of AFLP markers was observed
Map Data
T. turgidum, Messapia x dicoccoides, AFLP
Keyword
aflp
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-119-393
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||