GrainGenes Reference Report: PBR-120-354
Reference
PBR-120-354
Title
A new DNA extraction method for high-throughput marker analysis in a large-genome species such as Triticum aestivum
Journal
Plant Breeding
Year
2001
Volume
120
Pages
354-356
Author
Stein N Herren G Keller B
Abstract
Summary: Gene mapping and marker-assisted selection in complex, polyploid genomes still relies strongly on restriction fragment length polymorphism (RFLP) analysis, as conversion of RFLP to polymerase chain reaction (PCR) markers can be very difficult. DNA extraction in amounts suitable for RFLP analysis represents the most time-consuming and labour-intensive step in molecular marker analysis of plant populations. In this paper, a new flexible method for plant DNA extraction is presented. It allows a high-throughput of samples in a short time without the need for freezing or lyophilizing the plant material. The method allows the isolation of genomic DNA with a yield of approximately equal to 100 microgram for a minimal amount of 200 mg of leaf material. This is sufficient for work with large-genome plant species such as hexaploid wheat, where 20 microgram of genomic DNA are required for a single RFLP analysis
Keyword
amplification
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PBR-120-354
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||